Bio-One of Louisville decontamination and biohazard cleaning services

Compassion. Experience. Respect.


Kentucky Suicide, Crime Scene and Biohazard Cleanup

24/7 death, crime scene, suicide cleanup and other biohazard cleanup services from trusted, experienced, and discreet professionals serving Kentucky and the surrounding areas. Whether you’re in law enforcement, government, property management, or a local resident, Bio-One of Louisville has over a decade of experience serving you. We guarantee:
  • Complete discretion with unmarked vehicles and any special requests
  • Collaboration with your insurance companies
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Bio-One of Louisville
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502-572-0930
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"I called bio one after offering to help a family member and realizing it was too big of a job for just myself. Patricia came and did the walk through the day after I called and was so incredibly understanding and patient. She spoke with my uncle about his hobbies and other things which seemed to help ease his mind about the whole process. She is truly in the right field because Patricia carries so much compassion for the people she's cleaning for. Her and her team got everything done and cleaned in more than a timely manor. I was surprised at how fast everything moved. I can not recommend Bio One enough. They whole team was amazing and have definitely changed my uncles life and given him a second chance. Thank you Patricia. "
Christina Wofford
They did an amazing job! Helped me get my life back.
Mike Seiler
I can't thank Patricia and the Bio-One crew enough for their help. After the sudden passing of my sister I hired Bio One to clean her apartment and pack everything for donation. They did an outstanding job and really took a burden off my shoulders during a difficult time. Their work was impeccable but more importantly they did the job with respect and care. I can't recommend them enough.
Julia Mashburn
The Bio-One team did a fantastic job from the initial consultation to the day they arrived and started working. I couldn’t believe how much they accomplished in just one day. I highly recommend their services.
MissKaylee999
I cannot recommend this company highly enough. We have an elderly cat with some health issues who became quite confused about the location of his litter box in our basement. Patricia was able to come out to provide an estimate within a couple days of my call. In order to get rid of the smell, we had to rip out all the carpet and treat the concrete underneath. What a surprise to all of us when we discovered a second layer of glued down vinyl flooring underneath the carpet that ALSO had to be removed. Patricia and her team had only planned for a two-day job, but ended up out here three days to make sure they could fully address the issue - and they did! I appreciated them finding a way to get back out here so everything could be taken care of in the same week. The whole crew was incredibly professional, friendly, and amazing to work with. Their communication with me throughout the process was excellent, and I really feel like they went above and beyond in so many ways. For example, we had some mats under our exercise equipment that they weren't sure were salvageable because of the absorbency of the material. However, they took them out to the driveway to give them a really thorough scrub and power wash. In the end, we had to get rid of the mats, but I appreciated that they treated my home and belongings as thoughtfully and respectfully as they would have treated their own. Truly top notch service!
Abby Asfahl

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Mental Health First Aid LogoOsha Certified Mold Prevention, Remediation & RemovalFentanyl Remediation
Bio-One of Louisville decomposition / undiscovered death service

Decomposition / Undiscovered Death

When the unexpected happens, we are here for you.
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Bio-One of Louisville suicide cleanup service

Suicide Cleanup

We aim to help by cleaning with discretion and competence.
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Bio-One of Louisville feces / urine cleanup service

Feces / Urine Cleanup

We tailor every plan to each unique situation.
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Bio-One of Louisville odor removal service

Odor Removal

You shouldn’t have to settle with foul odors.
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Bio-One of Louisville homeless encampment cleanup service

Homeless Encampment Cleanup

A vital service to the community, done with compassion.
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Bio-One of Louisville blood spill cleanup service

Blood Spill Cleanup

Proper training is required to fully disinfect a blood spill.
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Bio-One of Louisville

    We're ReadY 24/7

    Work with us

    1. 1

      Establishing trust is key

      An experienced team member will introduce themselves, tour the property, and learn about your unique needs, expectations, and goals.
    2. 2

      We are mindful of personal property and valuables

      Our Bio-One of Louisville technicians make it a priority to try to find and save items of value such as wallets, keys, coin collections, legal documents, photo albums, baseball cards, or more.
    3. 3
      Your satisfaction is important
      We want to make sure you are satisfied and happy with our work. If for some reason something else needs to be done, we are here for you.

    Available 24 / 7 / 365

    We are here for you when you need us. For any Kentucky crime scene cleanup and biohazard decontamination situation, we are available 24/7. Whatever you need, know that on the other end of the line will be a supportive, understanding, and responsive Bio-One of Louisville team member.
    We are here for you when you need us the most.

    WHY CHOOSE Bio-One of Louisville?

    We are here for you when
    you need us the most.

    Our clients choose us for our proven expertise in providing prompt, professional crime scene clean up services and restoring property to its original condition. Bio-One of Louisville specializes in all types of clean up including blood cleaning, death cleaning, murder clean up, suicide clean up, crime scene clean up and homicide clean up in the KY Area and throughout Kentucky.
    Bio-One of Louisville services all types of trauma, distressed property, and biohazard scenes in communities throughout Kentucky Area. We are your crime scene cleaners dedicated to assisting law enforcement, public service agencies and property owners/managers in restoring property that has been contaminated as a result of crime, disaster or misuse.

    Your Kentucky Crime scene & Biohazard Cleaning TEAM

    Help first. Discretion always.

    As last responders, we discreetly remediate impacted 
    spaces so you can begin to move forward.
    Bio-One of Louisville owner, Patricia Smith
    Patricia Smith
    Owner
    Patricia's roots are in a small town in Central Wisconsin where she raised her six children, three boys and three girls. She considered it a great privilege to homeschool them and walk along side of them into adulthood. She then spent a few years running her own housekeeping business as well as working as a teacher's aide in the special ed department before recently partnering with Bio-One. Her background equips her to be professional and compassionate toward those needing the resources that this company provides.
    Learn more about me and my mission >

    The right
    team for the job.

    Making the call to a crime scene, biohazard, or decontamination cleaning company is often due to an overwhelming, traumatic, and life-changing situation. My team is ready to answer the call with professionalism, experience, and compassion.
    • Experienced and trained team
    • Clear, supportive communication
    • Comprehensive cleaning approach
    • Locally owned and operated
    Meet my team >
    Bio-One Colorado Team
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    jim owens, Jefferson County, 2/1/2025
    Easy to work with and very professional. Would recommend using their services for your cleaning needs.
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    William Hainline, Jefferson County, 1/1/2025
    Patricia and her crew were SO helpful, kind, virtuous and compassionate and caring. They held my hand through every step of the process and their service is not bad either! They are truly wonderful people and I would NOT want to go through that with ANYONE ELSE but then!
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    Jd Carruthers, Jefferson County, 11/1/2024
    I cannot overstate how pleased I am with the work I contracted with Bio One. The project I needed help with was a personally difficult circumstance for me since it involved a family member hoarding inside a condominium unit with surrounding neighbors. I had to call upon Bio One twice over the course of a year to complete a clean out of the unit, the second time involving remediation of decomposition after the death of my family member in the unit. Their professionalism and sensitivity to the difficulty of my circumstances were unparalleled. Patricia was compassionate, patient, reliable, and easy to deal with. I give the Bio One team the highest recommendation I could possibly express. Thank you!
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    Jackie Lambert, Jefferson County, 10/1/2024
    The Bio One of Louisville team came out to one of our rentals to assess a situation. They gave input and expertise and in the end, suggested a better resolve for my situation. That is top notch service! I appreciate it very much and look forward to using these guys again in the future.
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    Denise B, Jefferson County, 10/1/2024
    If I could give them 10 stars I would! Very professional, hard working employees who never stop moving and cleaning. They even did some extra little jobs that we didn’t ask for. Would recommend them to anyone who needs this type of service.
    decontamination and biohazard cleaning services reviewsCustomer Review Rating
    Crystal Herr, Jefferson County, 10/1/2024
    I can’t begin to tell you how empathetic, kind, patient and understanding Patricia and her team were. They helped a hurting family though a difficult time and were 1000% professional. They went way above and beyond expectations. They are saints. Would 1000% recommend!!!

    COMMUNITY

    We call Kentucky and the surrounding area home.

    Where we serve

    Bio-One of Louisville is owned and operated locally by Patricia Smith. Their office serves Kentucky and surrounding areas.

    Why we serve

    We are passionate about serving Kentucky and the surrounding communities with compassion, diligence, and professionalism. We are available 24/7 for your crime scene cleanup and biohazard decontamination needs.